Prof. Dr. Lars Nitschke
Lehrstuhl für Genetik
The role of Siglec proteins in B cell activation and autoimmunity
B cells express inhibitory receptors on the cell surface, which can suppress responses of the B cell antigen receptor (BCR). These inhibitory receptors control the strength of B cell activation. This control may also be important to maintain B cell tolerance, as many BCRs are autoreactive and can potentially trigger autoimmune responses. CD22 and Siglec-G are inhibitory receptors of the Siglec family that can bind to cell surface expressed sialic acids in specific linkages (Fig.1). Since sialic acids are abundantly expressed in vertebrates, but usually not in microorganisms they may act as self ligands by binding to Siglecs and suppressing B cell activation. By generation of new mouse models with mutated CD22- or Siglec-G-ligand binding domains it will be studied how ligand-binding controls the inhibitory activity of these molecules. Siglec-G seems to be a B1-cell specific inhibitory receptor, as loss of Siglec-G only affects the B1 cell population. In this project the regulation and selection of precursor cells into the B1 cell pool will be studied. We will also study the role of CD22 and Siglec-G in autoimmunity by crossing CD22- and Siglec-G-deficient mice into autoimmune-prone mouse strains, which develop a SLE-like disease. We will also address the question whether higher expression of CD22 and Siglec-G can prevent autoimmunity by generating new mouse models overexpressing these two proteins. These planned experiments will show how these two inhibitory receptors are involved in generating B cell tolerance and preventing autoimmunity.
Gruber, S., Hendrikx, T., Tsiantoulas, D., Ozsvar-Kozma, M., Goderle, L., Mallat, Z., Witztum, J.L., Shiri-Sverdlov, R., Nitschke, L., and Binder, C.J. (2016). Sialic Acid-Binding Immunoglobulin-like Lectin G Promotes Atherosclerosis and Liver Inflammation by Suppressing the Protective Functions of B-1 Cells. Cell Rep 14, 2348-2361.
Gasparrini, F., Feest, C., Bruckbauer, A., Mattila, P.K., Muller, J., Nitschke, L., Bray, D., and Batista, F.D. (2016). Nanoscale organization and dynamics of the siglec CD22 cooperate with the cytoskeleton in restraining BCR signalling. EMBO J 35, 258-280.
Müller, J., Lunz, B., Schwab, I., Acs, A., Nimmerjahn, F., Daniel, C., and Nitschke, L. (2015). Siglec-G deficiency leads to autoimmunity in aging C57BL/6 mice. J Immunol 195, 51-60.
Bökers, S., Urbat, A., Daniel, C., Amann, K., Smith, K.G., Espeli, M., and Nitschke, L. (2014). Siglec-G deficiency leads to more severe collagen-induced arthritis and earlier onset of lupus-like symptoms in MRL/lpr mice. J Immunol 192, 2994-3002.
Hutzler, S., Özgör, L., Naito-Matsui, Y., Kläsener, K., Winkler, T.W., Reth, M., and Nitschke, L. (2014). The ligand-binding domain of Siglec-G is crucial for its selective inhibitory function on B1 cells. J Immunol 192, 5406-5414.
Müller, J., and Nitschke, L. (2014). The role of CD22 and Siglec-G in B-cell tolerance and autoimmune disease. Nat Rev Rheumatol 10, 422-428.
Nitschke, L. (2014). CD22 and Siglec-G regulate inhibition of B-cell signaling by sialic acid ligand binding and control B-cell tolerance. Glycobiology 24, 807-817.
Müller, J., Obermeier, I., Wohner, M., Brandl, C., Mrotzek, S., Angermuller, S., Maity, P.C., Reth, M., and Nitschke, L. (2013). CD22 ligand-binding and signaling domains reciprocally regulate B-cell Ca2+ signaling. Proc Natl Acad Sci U S A 110, 12402-12407.